
Multimode AFM System (Bruker)
Scanning Probe Microscopies are key enabling techniques in Nanoscience and Nanotechnology, supporting a wide range of multidisciplinary activities. The LMA hosts various multipurpose AFM/STM heads that cover a broad range of applications under near ambient conditions. Our facility houses two Multimode AFMs from Bruker (originally fabricated by Veeco and then absorbed by Bruker). Both a Multimode V and a Multimode VIII offer high-quality imaging in contact and dynamic modes. The instruments are mounted on vibration isolation damping tables allowing the operation at very low noises. The samples surface can be held in ambient conditions, in well-defined gaseous atmospheres as well as be immersed in liquid (using a liquid-cell.). Sample temperature can be varied in a range of –22 to 200 °C
Key Features
- Highest environmental control;
- remarkable variety of AFM modes;
- PeakForce™ (Multimode VIII) and HarmoniX™ (Multimode V) for efficient quantitative nanomechanical characterization”;
- closed-loop scanner control for increased thermal/mechanical stability (Multimode V)


- What kind of information can be obtained with these instruments?
- Sample requirements
- Technical Specifications
The sample should be immobilized onto a flat substrate (for instance, biomolecules should be immobilized onto a mica surface via adsorption or via a covalent procedure).
The sample should exhibit a lower roughness than the range of the piezo scanner.
The size of the sample should be small enough to fit inside of the microscope, around 1 cm2 in surface and 0.5 cm in thickness.
Types of samples that can be studied with the environmental SPMs include:
- Biological samples (DNA, proteins and peptides; cells, viruses and bacteria; biological tissues, etc.).
- Organic and inorganic thin films.
- Gels and Polymers.
Environment: Air, gas, liquid and electrochemical cell
Temperature range: [-25, 200] ˚C
Scanner (exchangable): type J: 200 µm(X) x 200 µm(Y) x 5 µm(Z), type E: 12 µm x 12 µm x 3 µm, closed-loop: 90 µm(X) x 90 µm(Y) x 8µm(Z); PicoForce 40 µm(X) x 40 µm(Y) x 20 µm(Z)

Fig 1: Air topography of Rizobacteria

Fig 2: Graphene nanoflakes on mica

Fig 3: Topography and magnetic signal (phase contrast) from a focus ion beam fabricated nanostructure

Fig 4: Simultaneous Topography and electrical conductivity from nanoelectrodes.

Fig 5: Force spectroscopy of proteins with a biofunctionalized AFM tip