The Transmission Electron Microscope (TEM) JEM 1010 allows the study of the cellular ultrastructure in ultrafine sections (50-70 nm) obtained with an ultramicrotome. Likewise, using negative staining techniques it is possible to study viruses, bacteria, proteins and nanoparticle-biomolecule interaction systems. The cryotechnics allow greater preservation of the antigenicity for the labeling of proteins with antibodies conjugated with colloidal gold. For this purpose, a Leica AFS Cryosubstitution kit is available for the inclusion of samples in low temperature resins and a Leica Ultracut S Cryoultratragrometer for the obtaining of ultrafine sections in freezing.
What can be done with it?
- Cell characterization.
- Ultrastructure cell’s in plant and animals tissue studies.
- Localization and identification of specified biological substance at the ultrastructural level by inmunocytochemistery.
- Viruses reconigtion.
- Nanoparticles size determination.
- Studies of nanoparticles addition.
- Acceleration voltaje 100kV.
- LaB6 gun.
- Point resolution 0.35 nm.
- Megaview II camera.